Negatively Charged Peptide Nanofibrils from Immunoglobulin Light Chain Sequester Viral Particles but Lack Cell-Binding and Viral Transduction-Enhancing Properties

Positively charged naturally occurring or engineered peptide nanofibrils (PNF) are effective enhancers of lentiviral and retroviral transduction, an often rate-limiting step in gene transfer therapy approaches. These polycationic PNF thought to bridge the electrostatic repulsions between negatively membranes virions cells, thereby enhancing virion attachment infection target cells. Here, we analyzed PNF, which formed by AL1, that represents a fragment immunoglobulin light chain causes systemic AL amyloidosis. We found AL1 interact with viral particles comparable extent as positively PNF. However, lacked cell-binding activity, consequently, did not enhance infection. findings show capture cell binding mediated different mechanisms, offering avenues for design advanced selective functions.